Please find below the latest Ximbio product update. This month’s update includes nine new monoclonal antibodies available for license or purchase*.

This update also includes our featured enzyme! See below for more details.

For licensing and sales enquiries please email: enquiries@ximbio.com or call +44 (0)20 3469 6449.  Technical datasheets for Ximbio’s expanding research tool portfolio can be accessed at www.ximbio.com.
 
*Terms may vary with each product.
 
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Featured Product!
Positive control: Bovine ribonuclease B (RNase B)
Research area: Metabolism
PMID: 24069349, 22373601.
This fungal endo-glycosidase (ENGase) from Trichoderma viride will release “Man5GlcNAc to Man9GlcNAc oligosaccharides from RNase B, ManGlcNAc, Man3GlcNAc, Man5GlcNAc, Man6GlcNAc and Man8GlcNAc from ovalbumin, and high-mannose and oligomannose structures from yeast invertase.”1 However, this enzyme will “not act on fucosylated, hybrid, complex-type or bisecting N-acetyl-glucosamine-containing N-linked oligosaccharide structures from ovalbumin, IgG and fetuin.”1 The exclusive preference of this enzyme “for high mannose type oligosaccharides without substitutions is in contrast to the bacterial ENGases, Endo H and Endo F1, which additionally cleave hybrid structures, and also tolerate fucosylation in the case of Endo H.”1 This enzyme has an ~Mr of 35 kDa as determined by SDS-PAGE. 
 
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License
Applications: FACS
PMID: 11342414 , 9763568.
CD11A encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of alpha and beta chains. This L-domain containing alpha integrin combines with the beta 2 chain to form the integrin lymphocyte function-associated antigen-1 expressed on all leukocytes and this plays a central role in leukocyte intercellular adhesion through interactions with its ligands.
 
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License
Applications: IF WB
Reactivity: E.coli
E.coli BirA is a well-characterized multifunctional protein that either catalyses the transfer of biotin to biotin carboxyl carrier protein (BCCP), a acetyl-CoA carboxylase subunit or, alternatively, binds to the biotin operator resulting in transcriptional repression of the biotin operon. A mutant BirA (R118G) shows loss of DNA binding ability and promiscuity of substrate biotinylation. These properties of BirA R118G were used to develop an in vivo proximity labelling technique that identifies potential interacting proteins (BioID). In the BioID method, a protein of interest is tagged with BirA R118G and expressed in live cells. Addition of the biotin to the culture medium results in biotinylation of proteins in vicinity of the bait. The biotinylated proteins can then be affinity purified with biotin binding proteins such as strepavidin or avidin even under harsh denaturing conditions and subsequently identified by mass spectrometry or immunoblot analysis.
 
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License
Applications: IF WB
Reactivity: Human
A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.
 
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License
Applications: IF WB
Reactivity: Human
A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.
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License
Applications: ELISA FACS IHC IP WB
Reactivity: Rat
Carcinoembryonic antigen related cell adhesion molecules (CEACAM) (CD66a) belong to the immunoglobulin superfamily. They are highly glycosylated proteins with the typical N-terminal variable Ig-like domain followed by 0-6 constant Ig-like domains. A hydrophobic transmembrane domain with a cytoplasmic tail anchors CEACAM1 to the cell membrane. CEACAM1 is known as an epithelial tumour suppressor and an angiogenic growth factor.
 
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License
Applications: ELISA FACS IHC IF IP WB
Reactivity: Rat
PMID: 15909305 , 10728682.
Carcinoembryonic antigen related cell adhesion molecules (CEACAM) (CD66a) belong to the immunoglobulin superfamily. They are highly glycosylated proteins with the typical N-terminal variable Ig-like domain followed by 0-6 constant Ig-like domains. A hydrophobic transmembrane domain with a cytoplasmic tail anchors CEACAM1 to the cell membrane. CEACAM1 is known as an epithelial tumour suppressor and an angiogenic growth factor.
 
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License
Applications: EM IF
Reactivity: Human
PMID: 27802546 , 7524609
LH24 targets the basement membrane zone of keratinocytes, specifically Type VII Collagen non-collagenous domain 2. Type VII collagen and anchoring fibril deficiencies in skin biopsy samples were confirmed by immunofluorescence microscopy and immunoelectron microscopy using the LH24 antibody.

 
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License
Applications: ELISA FACS IHC IP WB
Reactivity: Human
PMID: 24743304 , 23941132
CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM8 is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM8, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CEACAM6.
 
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License
Applications: ELISA FACS IF IP WB
Reactivity: Human
CEACAM5 is a glycoprotein involved in cell adhesion and intracellular signaling. It is normally produced during fetal development in the gut, and its production stops before birth. CEA is re-expressed in increased amounts in Intestinal Carcinomas and several other tumors. 
 
 

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