Please find below the latest Ximbio product update. This month’s update includes seventeen new monoclonal antibodies available for license or purchase*.

This update includes our featured polyclonal antibody! See below for more details.

For licensing and sales enquiries please email: [email protected] or call +44 (0)20 3469 6449.  Technical datasheets for Ximbio’s expanding research tool portfolio can be accessed at www.ximbio.com.
 
*Terms may vary with each product.
 
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Applications: IF WB
Reactivity: Human, Mouse
Anti-phospho-Sufu polyclonal antibody recognises wild type sufu which is phosphorylated at serine 352. The antibody specificity for the phosphorylated version has been confirmed; it does not react against a non-phosphorylated version of Sufu which has serine 352 substituted by alanine.
Sufu is a negative regulator in the hedgehog signaling pathway which directs cell proliferation and patterning during embryogenesis. Sufu down-regulates GLI1- and GLI2-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. Required for normal embryonic development. Sufu is ubiquitous in adult tissues.
Heterozygous loss of Sufu, in conjunction with the loss of p53, leads to the development of medulloblastoma and rhabdomyosarcoma. Somatic Sufu mutations have been identified in multiple other malignancies, including prostate cancer.
Featured Product!
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Applications: ELISA WB
Reactivity: Rainbow Trout
Three interleukin 4 molecules are found in rainbow trout; IL4-4B1, IL4-4B2, IL4/13A. This antibody is specific to IL4B1. 
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Applications: ELISA IHC WB
PMID: 20083366, 19850673, 19250787, 18402977.
IgG is a type of antibody, has two antigen binding sites, and represents approximately 75% of serum antibodies in humans. IgG is the most common type of antibody found in the circulation and they are created and released by plasma B cells.
License
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Applications: IHC  ELISA WB
Reactivity: Gilthead seabream, Meagre and Lumpsucker 
Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. This Anti-IgT antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines, especially mucosal vaccines. Target species: Meagre (Argyrosomus regius), gilt-head (sea) bream (Sparus aurata), Lumpsucker (Cyclopteridae), Large yellow croaker (Larimichthys crocea).
License
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Applications: ChIP ELISA IHC EMSA
Reactivity: Human
ELAV-like protein 1 or HuR (human antigen R) is a protein that in humans is encoded by the ELAVL1 gene. The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It stabilizes mRNAs and thereby regulates gene expression.
A popular technique is to use anti-HuR antibodies to pull out interacting mRNAs and thereby analyse gene expression in a particular tissue. Monoclonal antibody 4C8 was selected by its ability to bind to the HuR/mRNA complex and thus is very useful for EMSA, ChIP and CLIP studies.
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Applications: ELISA FACS IHC WB
Reactivity: Human
ELAV-like protein 1 or HuR (human antigen R) is a protein that in humans is encoded by the ELAVL1 gene. The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It stabilizes mRNAs and thereby regulates gene expression. Mab 19F12 is a well cited antibody used in the identification of HuR.
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Applications: ChIP IHC WB
Reactivity: Human
The determination of Dicer expression is of critical importance in the diagnosis of human cancer. Dicer can be used to identify whether tumors are present within the body based on the expression level of the enzyme. A study showed that many patients that had cancer had decreased expression levels of Dicer. The same study showed that lower Dicer expression correlated with lower patient survival length. Monoclonal 13D6 is the classic antibody for the analysis of Dicer. 
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Applications: ChIP ELISA IHC IP WB
Reactivity: Human
The determination of Dicer expression is of critical importance in the diagnosis of human cancer. Dicer can be used to identify whether tumors are present within the body based on the expression level of the enzyme. A study showed that many patients that had cancer had decreased expression levels of Dicer. The same study showed that lower Dicer expression correlated with lower patient survival length. Mouse monoclonal 4A6 is of particular utility for IHC on FFPE sections.
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Applications: ELISA IHC WB
Reactivity: Human, Mouse
TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is a receptor on some percentage of T cells and Natural Killer Cells (NK). It is also identified as WUCAM and Vstm3. TIGIT binds with high affinity to the poliovirus receptor (PVR), also known as CD155, which causes increased secretion of IL-10 and decreased secretion of IL-12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Research has shown that TIGIT-Fc fusion protein could interact with PVR on dendritic cells and increase its IL-10 secretion level/decrease its IL-12 secretion level under LPS stimulation, and also inhibit T cell activation in vivo. TIGIT's inhibition of NK cytotoxicity can be blocked by antibodies against its interaction with PVR and the activity is directed through its ITIM domain.
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icon-01_Antibodies.jpg Anti-PD-L1 [29E.2A3]  
Applications: FACS IHC Fn
Reactivity: Human
PMID: 12538684
PD-L1 is notably expressed on macrophages. In the mouse, it has been shown that classically activated macrophages (induced by type I helper T cells or a combination of LPS and interferon-gamma) greatly upregulate PD-L1. Alternatively, macrophages activated by IL-4 (alternative macrophages), slightly upregulate PD-L1, while greatly upregulating PD-L2. It has been shown by STAT1-deficient knock-out mice that STAT1 is mostly responsible for upregulation of PD-L1 on macrophages by LPS or interferon-gamma, but is not at all responsible for its constitutive expression before activation in these mice.
It appears that upregulation of PD-L1 may allow cancers to evade the host immune system. An analysis of 196 tumor specimens from patients with renal cell carcinoma found that high tumor expression of PD-L1 was associated with increased tumor aggressiveness and a 4.5-fold increased risk of death. Many PD-L1 inhibitors are in development as immuno-oncology therapies and are showing good results in clinical trials.
 
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Applications: ELISA IHC WB
Reactivity: Human
The PD-1 protein is an inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. A new class of drugs that block PD-1, the PD-1 inhibitors, activate the immune system to attack tumors and are therefore used to treat cancer.
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Applications: ELISA IHC WB
Reactivity: Mouse
The monoclonal antibody 11F4 recognizes mouse CD119 also known as IFN gamma receptor 1. CD119 heterodimerizes with IFN gamma receptor 2 (AF-1) forming the Class II cytokine receptor. The ligand IFN gamma binds IFN gamma receptor 1 subunit but signal transduction transpires via the IFN gamma receptor 2 subunit. The receptor is involved in host defense, expression is broad including B cells, NK cells, macrophages and dendritic cells as well as other non-hematopoietic cell types.
Purchase
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Applications: ChIP IHC WB
Reactivity: Human
Affinity purified biotinylated antibody specifically directed against HuD E, the mutant HuD neoantigen expressed in small cell lung carcinoma (SCLC), melanoma and neuroblastoma.
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Applications: ChIP ELISA IHC IP WB
The green fluorescent protein (GFP) is a protein composed of 238 amino acid
residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.
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Applications: ChIP ELISA IHC WB
Reactivity: Human
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. Monoclonal antibody 5C12 is a high affinity mouse monoclonal directed against GST. It has been independently validated by the Online Antibodies validation program and can detect at least 1ng of GST protein. Its high affinity makes it the antibody of choice for ChIP studies.
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Applications: ChIP ELISA IHC IF IP WB
Reactivity: Human
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is N-EQKLISEEDL-C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.
It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway. Monoclonal 5A5 was selected by its excellent performance in ChIP analyses.
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